CytoNorm note

R code to demonstrate the functionalities of CytoNorm2.0.

Demonstrated use cases

• Normalize without using technical replicates or controls
• Normalize towards another model, batch or goal distribution
• Normalize markers without including them in the clustering step

Visualizations

• Waterfall plot
• FlowSOMmary
• Splines
• Densities

Data

The dataset that was used to show all functionalities is available on FlowRepository (ID: FR-FCM-Z6UT).