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Added Minimap2 alignment #1486

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EgorGuga
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Updated version of pull-request #1459

Overview

In this pull-reqest, we added the ability to align reads using the minimap2 tool. The use of the tool is justified by the fact that this tool is popular and is used in commercial solutions, particularly in MegaBOLT.

P.S.
As long as the sort_bam variable is hardcoded as true a call of the minimap2 tool will not cause any problems.

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
  • If necessary, also make a PR on the nf-core/sarek branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker --outdir <OUTDIR>).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

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github-actions bot commented Apr 26, 2024

nf-core lint overall result: Passed ✅ ⚠️

Posted for pipeline commit f2c1753

+| ✅ 201 tests passed       |+
#| ❔  12 tests were ignored |#
!| ❗   3 tests had warnings |!

❗ Test warnings:

  • pipeline_todos - TODO string in main.nf: Optionally add in-text citation tools to this list.
  • pipeline_todos - TODO string in main.nf: Optionally add bibliographic entries to this list.
  • pipeline_todos - TODO string in main.nf: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled!

❔ Tests ignored:

  • files_exist - File is ignored: .github/workflows/awsfulltest.yml
  • files_exist - File is ignored: .github/workflows/awstest.yml
  • files_exist - File is ignored: conf/modules.config
  • files_unchanged - File ignored due to lint config: .github/PULL_REQUEST_TEMPLATE.md
  • files_unchanged - File ignored due to lint config: assets/nf-core-sarek_logo_light.png
  • files_unchanged - File ignored due to lint config: docs/images/nf-core-sarek_logo_light.png
  • files_unchanged - File ignored due to lint config: docs/images/nf-core-sarek_logo_dark.png
  • files_unchanged - File ignored due to lint config: .gitignore or .prettierignore
  • actions_ci - actions_ci
  • actions_awstest - 'awstest.yml' workflow not found: /home/runner/work/sarek/sarek/.github/workflows/awstest.yml
  • template_strings - template_strings
  • modules_config - modules_config

✅ Tests passed:

Run details

  • nf-core/tools version 2.14.1
  • Run at 2024-10-04 07:59:27

@maxulysse maxulysse added this to the 3.5 milestone May 7, 2024
@maxulysse maxulysse changed the title Added Minimap2 alignment (3.4.1) Added Minimap2 alignment May 7, 2024
@maxulysse
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@nf-core-bot fix linting pretty please 🙏

@EgorGuga
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EgorGuga commented Jun 5, 2024

Please test with the last commit

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Thanks for the efforts @EgorGuga

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@nf-core-bot fix linting pretty please 🙏

docs/usage.md Outdated Show resolved Hide resolved
@@ -733,6 +733,13 @@
"hidden": true,
"help_text": "If you use AWS iGenomes, this has already been set for you appropriately."
},
"minimap2": {
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I would have named that parameter minimap2_index.

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I think we have bwa and bwamem2, so I'm happy with just minimap2

@FriederikeHanssen FriederikeHanssen removed this from the 3.5 milestone Aug 19, 2024
maxulysse and others added 2 commits October 4, 2024 09:55
<!--
# nf-core/sarek pull request

Many thanks for contributing to nf-core/sarek!

Please fill in the appropriate checklist below (delete whatever is not
relevant).
These are the most common things requested on pull requests (PRs).

Remember that PRs should be made against the dev branch, unless you're
preparing a pipeline release.

Learn more about contributing:
[CONTRIBUTING.md](https://github.com/nf-core/sarek/tree/master/.github/CONTRIBUTING.md)
-->

## PR checklist

- [ ] This comment contains a description of changes (with reason).
- [ ] If you've fixed a bug or added code that should be tested, add
tests!
- [ ] If you've added a new tool - have you followed the pipeline
conventions in the [contribution
docs](https://github.com/nf-core/sarek/tree/master/.github/CONTRIBUTING.md)
- [ ] If necessary, also make a PR on the nf-core/sarek _branch_ on the
[nf-core/test-datasets](https://github.com/nf-core/test-datasets)
repository.
- [ ] Make sure your code lints (`nf-core lint`).
- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker
--outdir <OUTDIR>`).
- [ ] Check for unexpected warnings in debug mode (`nextflow run .
-profile debug,test,docker --outdir <OUTDIR>`).
- [ ] Usage Documentation in `docs/usage.md` is updated.
- [ ] Output Documentation in `docs/output.md` is updated.
- [ ] `CHANGELOG.md` is updated.
- [ ] `README.md` is updated (including new tool citations and
authors/contributors).
@EgorGuga
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EgorGuga commented Dec 6, 2024

@nf-core-bot fix linting pretty please 🙏

EgorGuga and others added 2 commits December 6, 2024 17:45
applied suggested change in docs/usage.md

Co-authored-by: Anders Sune Pedersen <[email protected]>
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Warning

Newer version of the nf-core template is available.

Your pipeline is using an old version of the nf-core template: 3.0.2.
Please update your pipeline to the latest version.

For more documentation on how to update your pipeline, please see the nf-core documentation and Synchronisation documentation.

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5 participants