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Cat fastq feature #179

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Cat fastq feature #179

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SpikyClip
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@SpikyClip SpikyClip commented Jan 25, 2025

Changes

  • Adds the cat_fastq module which allows merging of resequenced fastq files before samples are processed downstream, fixes No cat_fastq? Sample with multiple run are not handled #148.
  • The plugin nf-schema was also added and samplesheet tests introduced (facilitated by the plugin's function samplesheetToList).
  • Adds test profiles to test fastq concatenation (test_fastq_cat), also included in test_full. Original test sample data was split with seqkit into two parts.
  • schema_input.json updated to 2020-12 json standard.

Potential Issues

  • Cannot update nextflow_schema.json to 2020-12, even when following the migration guide. For some reason doing so results in the error message:
    ERROR ~ #: could not determine version
    
    However the pipeline still runs fine even with nextflow_schema.json, it just fails nf-core pipeline lint.

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs- [ ] If necessary, also make a PR on the nf-core/hlatyping branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker --outdir <OUTDIR>).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

apeltzer and others added 10 commits October 18, 2022 13:16
Has more useful parsing/validation methods, which would be relevant as this pipeline takes multiple possible data types (i.e. bams and/or fastqs).
meta.id is appended to front of list to facilitate joining by `.groupTuple()`, and is eventually dropped by `checkSamplesAfterGrouping` which returns `[meta, input_files]` as expected by rest of pipeline.
@SpikyClip SpikyClip self-assigned this Jan 25, 2025
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This PR is against the master branch ❌

  • Do not close this PR
  • Click Edit and change the base to dev
  • This CI test will remain failed until you push a new commit

Hi @SpikyClip,

It looks like this pull-request is has been made against the SpikyClip/hlatyping master branch.
The master branch on nf-core repositories should always contain code from the latest release.
Because of this, PRs to master are only allowed if they come from the SpikyClip/hlatyping dev branch.

You do not need to close this PR, you can change the target branch to dev by clicking the "Edit" button at the top of this page.
Note that even after this, the test will continue to show as failing until you push a new commit.

Thanks again for your contribution!

@SpikyClip SpikyClip added the enhancement New feature or request label Jan 25, 2025
@SpikyClip SpikyClip changed the base branch from master to dev January 25, 2025 03:00
Apologies, should have worked off `dev` to begin with.
@christopher-mohr
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Thanks @SpikyClip, I will have a look later today. 👍

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No cat_fastq? Sample with multiple run are not handled
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