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Registration README file updated
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behnam-yousefi committed Dec 10, 2024
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8 changes: 4 additions & 4 deletions notebooks/03_image_registration/README.md
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# H&E-DAPI registration

For each sample,
1. Cut the DAPI via ```seperate_samples_LEVEL1.ipynb``` and save TIFF and anndata objects.
1. Cut the DAPI via ```seperate_samples.ipynb``` and save TIFF and anndata objects.
2. Cut H&E images via Fiji.
3. Do the registarion with ```registration_final_elastix.ipynb```
3. Do the registarion with ```main.py```

As for visualization, we need to read the anndata for each sample, then attach the corresponding registared H&E image to it as in ```plot_example.ipynb```.


# Phenocycler-DAPI Registration

1. Cut the DAPI via ```seperate_samples_LEVEL1_slide.ipynb```
1. Cut the DAPI via ```seperate_samples_phenocycler.ipynb```
2. Cut Phenocycler image ```Xenium Slide_Scan1.qptiff``` via Fiji.
3. Do the registarion with ```registration_final_elastix_phenocycler.ipynb```
3. Do the registarion with ```main.py```


**Cut Phenocycler image:**
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