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Count number of mapped reads per reference in SAM files (often for RNA-Seq experiments)

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sam2counts.py - Convert SAM mapping results to reference sequence counts

Requirements

Usage

Bare minimum:

python sam2counts.py sample_1.sam sample_2.sam

This produces a tab-delimited file named counts.txt that contains the counts for each reference. If you prefer to have it named something else:

python sam2counts.py -o s1_to_s2.txt sample_1.sam sample_2.sam

Or use a different delimiter:

python sam2counts.py -o s1_to_s2.csv -d, sample_1.sam sample_2.sam

Todo

Currently there is code to indicate if mapped reads map with 0 quality (i.e. map to multiple locations). The best output for this is still being decided, and this is not entirey implemented

Furthermore, this code was tested fairly heavily, but I make no guarantees.

License and Contact

If you have questions regarding this code, please contact Vince Buffalo at [email protected] (with the poly-A tail removed). All code copyright Vince Buffalo, 2010.

License: GNU General Public License

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Count number of mapped reads per reference in SAM files (often for RNA-Seq experiments)

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