proks_et_al_2023

Complemetary analysis to nf-core/marsseq.

Dataset available at

nf-core/marsseq

module load java/11.0.15 nextflow/22.10.6 singularity/3.8.0

sh nf-core_tower.sh mm10_ref nextflow run $PIPELINE_PATH/nf-core-marsseq \
  --input $DATA_PATH/SB26/design.csv \
  --build_references \
  --genomes_base /scratch/Brickman/references \
  --genome mm10

sh nf-core_tower.sh SB26 nextflow run $PIPELINE_PATH/nf-core-marsseq \
  --input $DATA_PATH/SB26/design.csv \
  --genomes_base /scratch/Brickman/references \
  --genome mm10

sh nf-core_tower.sh SB28 nextflow run $PIPELINE_PATHnf-core-marsseq \
  --input $DATA_PATH/SB28/design.csv \
  --genomes_base /scratch/Brickman/references \
  --genome mm10

MARS-seq2.0

module load miniconda/latest

source activate ngs
cookiecutter https://github.com/brickmanlab/mars-seq2-inhouse

source activate mars
python helpers/prepare.py --input $PIPELINE_PATH/pipeline/SB26/ --output test_data

# split cells per Batch
scripts/split_fastqs.sh test_data/raw_reads/SB26/orig_files/ test_data/raw_reads/SB26/ 4000000
scripts/split_fastqs.sh test_data/raw_reads/SB28/orig_files/ test_data/raw_reads/SB28/ 4000000

# run pipeline
scripts/run_pipeline_locally.sh test_data