diff --git a/mypy/semanal.py b/mypy/semanal.py
index bad2dfbefae0..4bf342c1e128 100644
--- a/mypy/semanal.py
+++ b/mypy/semanal.py
@@ -2369,8 +2369,11 @@ def analyze_unbound_tvar_impl(
             assert isinstance(sym.node, TypeVarExpr)
             return t.name, sym.node
 
-    def find_type_var_likes(self, t: Type) -> TypeVarLikeList:
+    def find_type_var_likes(
+        self, t: Type, *, include_bound_tvars: bool = False
+    ) -> TypeVarLikeList:
         visitor = FindTypeVarVisitor(self, self.tvar_scope)
+        visitor.include_bound_tvars = include_bound_tvars
         t.accept(visitor)
         return visitor.type_var_likes
 
@@ -5036,9 +5039,15 @@ def analyze_value_types(self, items: list[Expression]) -> list[Type]:
         result: list[Type] = []
         for node in items:
             try:
-                analyzed = self.anal_type(
-                    self.expr_to_unanalyzed_type(node), allow_placeholder=True
-                )
+                unanalyzed_type = self.expr_to_unanalyzed_type(node)
+                if self.find_type_var_likes(unanalyzed_type, include_bound_tvars=True):
+                    self.fail(
+                        "TypeVar constraint type cannot be parametrized by type variables", node
+                    )
+                    result.append(AnyType(TypeOfAny.from_error))
+                    continue
+
+                analyzed = self.anal_type(unanalyzed_type, allow_placeholder=True)
                 if analyzed is None:
                     # Type variables are special: we need to place them in the symbol table
                     # soon, even if some value is not ready yet, see process_typevar_parameters()
diff --git a/mypy/typeanal.py b/mypy/typeanal.py
index daf7ab1951ea..930cc46e44d4 100644
--- a/mypy/typeanal.py
+++ b/mypy/typeanal.py
@@ -2558,6 +2558,7 @@ def __init__(self, api: SemanticAnalyzerCoreInterface, scope: TypeVarLikeScope)
         self.has_self_type = False
         self.seen_aliases: set[TypeAliasType] | None = None
         self.include_callables = True
+        self.include_bound_tvars = False
 
     def _seems_like_callable(self, type: UnboundType) -> bool:
         if not type.args:
@@ -2583,7 +2584,7 @@ def visit_unbound_type(self, t: UnboundType) -> None:
         if (
             node
             and isinstance(node.node, TypeVarLikeExpr)
-            and self.scope.get_binding(node) is None
+            and (self.scope.get_binding(node) is None or self.include_bound_tvars)
         ):
             if (name, node.node) not in self.type_var_likes:
                 self.type_var_likes.append((name, node.node))
diff --git a/test-data/unit/semanal-errors.test b/test-data/unit/semanal-errors.test
index 33c8f9b80aa0..16b35be2deaa 100644
--- a/test-data/unit/semanal-errors.test
+++ b/test-data/unit/semanal-errors.test
@@ -1060,6 +1060,19 @@ S = TypeVar('S', covariant=True, contravariant=True) \
     # E: TypeVar cannot be both covariant and contravariant
 [builtins fixtures/bool.pyi]
 
+[case testInvalidTypevarArgumentsGenericConstraint]
+from typing import Generic, List, TypeVar
+
+T = TypeVar("T")
+
+Bad1 = TypeVar("Bad1", int, T)              # E: TypeVar constraint type cannot be parametrized by type variables
+Bad2 = TypeVar("Bad2", int, List[T])        # E: TypeVar constraint type cannot be parametrized by type variables
+Bad3 = TypeVar("Bad3", int, List[List[T]])  # E: TypeVar constraint type cannot be parametrized by type variables
+def f(x: T) -> None:
+    Bad4 = TypeVar("Bad4", int, List[T])    # E: TypeVar constraint type cannot be parametrized by type variables
+class C(Generic[T]):
+    Bad5 = TypeVar("Bad5", int, List[T])    # E: TypeVar constraint type cannot be parametrized by type variables
+
 [case testInvalidTypevarValues]
 from typing import TypeVar
 b = TypeVar('b', *[int]) # E: Unexpected argument to "TypeVar()"