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/usr/bin/activate: No such file or directory #1485

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gtmvish opened this issue Jan 10, 2025 · 0 comments
Open

/usr/bin/activate: No such file or directory #1485

gtmvish opened this issue Jan 10, 2025 · 0 comments
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bug Something isn't working

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@gtmvish
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gtmvish commented Jan 10, 2025

Description of the bug

I am getting error "Command error:
.command.run: line 317: /usr/bin/activate: No such file or directory"

Command used and terminal output

nf-core/rnaseq 3.18.0
------------------------------------------------------
Input/output options
  input      : /home/lus03/gtmvish/work/wheat/nfcore/samplesheet.csv
  outdir     : /home/lus03/gtmvish/work/wheat/nfcore/result/

Reference genome options
  fasta      : /home/lus03/gtmvish/work/wheat/ref_seqs/Triticum_aestivum.IWGSC.59.dna.toplevel.fa.gz
  gtf        : /home/lus03/gtmvish/work/wheat/ref_seqs/Triticum_aestivum.IWGSC.59.gtf.gz

Core Nextflow options
  revision   : master
  runName    : cheeky_einstein
  launchDir  : /home/lus03/gtmvish/work/wheat/nfcore
  workDir    : /home/lus03/gtmvish/work/wheat/nfcore/work
  projectDir : /home/lus03/gtmvish/.nextflow/assets/nf-core/rnaseq
  userName   : gtmvish
  profile    : conda
  configFiles:

!! Only displaying parameters that differ from the pipeline defaults !!
------------------------------------------------------
* The pipeline
    https://doi.org/10.5281/zenodo.1400710

* The nf-core framework
    https://doi.org/10.1038/s41587-020-0439-x

* Software dependencies
    https://github.com/nf-core/rnaseq/blob/master/CITATIONS.md

Execution cancelled -- Finishing pending tasks before exit
-[nf-core/rnaseq] Pipeline completed with errors-
ERROR ~ Error executing process > 'NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (sample2)'

Caused by:
  Process `NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (sample2)` terminated with an error exit status (1)


Command executed:

  printf "%s %s\n" TWM-89_1.fq.gz sample2_raw_1.gz TWM-89_2.fq.gz sample2_raw_2.gz | while read old_name new_name; do
      [ -f "${new_name}" ] || ln -s $old_name $new_name
  done

  fastqc \
      --quiet \
      --threads 6 \
      --memory 6144 \
      sample2_raw_1.gz sample2_raw_2.gz

  cat <<-END_VERSIONS > versions.yml
  "NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC":
      fastqc: $( fastqc --version | sed '/FastQC v/!d; s/.*v//' )
  END_VERSIONS

Command exit status:
  1

Command output:
  (empty)

Command error:
  .command.run: line 317: /usr/bin/activate: No such file or directory

Work dir:
  /home/lus03/gtmvish/work/wheat/nfcore/work/be/204d8b836f1c6456a83b5be13bb96c

Tip: view the complete command output by changing to the process work dir and entering the command `cat .command.out`

 -- Check '.nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting

 -- Check '.nextflow.log' file for details

Relevant files

logs.zip

System information

nextflow version 24.10.3
hardware: hpc
executor: PBS torque
profile conda
os CentOS Linux release 7.6
Version of nf-core/rnaseq 3.18.0

@gtmvish gtmvish added the bug Something isn't working label Jan 10, 2025
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