Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Suggested addition: a PCA illustrating sample/condition/replicates #166

Open
jeremymsimon opened this issue Jul 5, 2024 · 0 comments
Open
Labels
enhancement Improvement for existing functionality

Comments

@jeremymsimon
Copy link

Description of feature

Maybe this isn't the typical case for most users, but my experiment has multiple replicates of treatment and control. If I wanted to check how concordant my replicates were, and how separated my treatment from control conditions were, I'd likely attempt a PCA based on the raw or normalized counts produced by MAGeCK, coloring the points both by sample and condition (similar to DESeq2 output in nf-core/rnaseq). This could also help identify whether any sample(s) were outliers compared to others, which would be supported by the countsummary table--ie perhaps one sample was an outlier from others in PC space and also has a lower-than-usual mapping rate or very high Gini index etc.

I'd also plot the cross-sample pairwise correlations of normalized counts as a (n * n) heatmap, which likely also would be a useful output

@jeremymsimon jeremymsimon added the enhancement Improvement for existing functionality label Jul 5, 2024
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
enhancement Improvement for existing functionality
Projects
None yet
Development

No branches or pull requests

1 participant