This example shows how to use skeletonisation to measure the length of filamentous objects.
Segmentation of DNA from Atomic Force Microscope (AFM) images. (Left) Raw images showing linearised DNA deposited onto mica surface. (Right) Detected DNA molecules colour-coded according to whether they are single molecules (cyan) or are comprised of multiple overlapping molecules (magenta). Molecules on the image edge are automatically discarded.
This example introduces the following concepts:
- Using skeletonisation to measure the length of filamentous objexts
- Extended parent-child relationships (i.e. grandparent-grandchild)
- Partner-partner relationships
- Filtering objects based on their number of relationships with a specific class
- Moving filtered objects to a new object collection