This example shows how to track previously-detected objects cross multiple frames.
Key stages in detection and tracking of nuclei. (Left) Raw images showing nuclei over time. (Middle) Centroids of detected nuclei highlighted with orange spots. (Right) Trajectories of tracked nuclei represented by lines, colour-coded according to instantaneous velocity.
This example introduces the following concepts:
- Using the "Spot detection" module
- Using the "Track objects" module
- Making motion measurements for tracked objects
- Parent-child relationships in the context of tracked objects
- Filtering objects based on number of associated children
- Rendering tracks as overlays
Note: For this example, we're using a timelapse of yolk syncytial layer (YSL) nuclei from the Cell Image Library (doi: 10.7295/W9CIL11813). This timeseries was made available by Mark Cooper through Zebrafish - The Living Laboratory and is released under a CC BY-NC-SA 3.0 license.