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PTU_Writer

FIJI plugin generating PicoQuant ptu FLIM TTTR image file given a stack with lifetimes.
Reverse-engineering of PTU_Reader plugin.

How to install plugin

  1. You need to download and install FIJI on your computer first.
  2. Download PTU_Writer_...jar and place it in the "plugins" folder of FIJI.
  3. Plugin will appear as PTU_Writer in FIJI's Plugins menu.

How to run plugin

  1. Click PTU_Writer line in ImageJ's Plugins menu.
  2. In the following dialog select a TIF Z-stack, where Z corresponds to the "lifetime counts". Only 8- and 16-bit single channel stacks are supported for now.
  3. Plugin will ask for "time resolution" metadata parameters:
    dialog
    where TTResult_SyncRate is the laser repetition rate (in Hz) and
    MeasDesc_Resolution is the time between "lifetime counts", i.e. time between two neighboring input z-slices (in ps).
  4. In a new (last) dialog choose the folder and name for the output PTU file.

Updates history

v.0.0.3 (2024.12) Added extra tags, now can be loaded via ptufiles.
v.0.0.2 (2024.11) Added extra tags, now can be loaded via readPTU_FLIM.
v.0.0.1 (2024.11) First release.

Developed in Cell Biology group of Utrecht University.
Email katpyxa @ gmail.com for any questions/comments/suggestions.