Error in ScaleData(): ! No layer matching pattern 'data' found. Please run NormalizeData and retry Run rlang::last_trace() to see where the error occurred.

[Noralii]

Error in ScaleData():
! No layer matching pattern 'data' found. Please run NormalizeData and retry
Run rlang::last_trace() to see where the error occurred.

[xiaoli-gg]

Dear friends:
I am also confused by this Error. Have you solved this problem?
Hope to get your advice.
Thanks
Mengpan Li

[Tang-RH]

The error may be coused by Seurat V5, please check this answer:
#617 (comment)
Using the following command to convert the seurat object format to v3 before running seems to work temporarily:

options("Seurat.object.assay.version" = "v3")

[xiaoli-gg]

Dear friends, thanks for your advice.