Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

CNVs in the reference cells #663

Open
NaCo-Pt opened this issue May 7, 2024 · 1 comment
Open

CNVs in the reference cells #663

NaCo-Pt opened this issue May 7, 2024 · 1 comment

Comments

@NaCo-Pt
Copy link

NaCo-Pt commented May 7, 2024

first thank you for this amazing tool. Second, I have very basic knoledge of coding so bear with me

infercnv

I have used 2 cell types as references, CAFs and immune cells. Wihtin immune cells I can clearly see in the HM some cells with a CNV profile resembilg the tumor cells (observations bellow).
Does this mena I have some cells that were wrongly clustered with immune cells and could actually be tumor cells, or some doublets inside my immune cell cluster?
How can I identify which cells are those so when I go back to my seurat object I can isolate those cells and maybe exclude them from the analysis?

Another question, I have some cluster with a very reduced cell number and I cant visualize it well, like tje epithelial_cells. How cna I partioned my inferCNV heatmap to see just some clusters on the observation cells?

kind regards

@kiwipeel
Copy link

You can use some tumor related markers to isolate those cells among reference cells by subsetting based on the umi counts.

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

2 participants