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After "WaitingForReturnCode ", pipeline stuck #105
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hi,
If you rerun the original command, it should resume where it left off. The
workflow runner (cromwell) sometimes takes a little while to go to the next
step but it usually doesn't get completely stuck. It's an industry-strength
workflow runner.
best,
~b
…On Tue, Jan 11, 2022 at 4:19 PM jaewon-cho ***@***.***> wrote:
I was running the ctat-mutation pipeline with singularity.
I have done the "test" data before
Here is my script:
singularity exec -e -B
${CTAT_GENOME_LIB}:/data/hemberg/jaewon/ctat/new5/GRCh37_gencode_v19_CTAT_lib_Mar012021.plug-n-play/ctat_genome_lib_build_dir/
~/ctat_mutations.v3.2.0.simg
~/ctat-mutations-CTAT-Mutations-v3.2.0/ctat_mutations --left
test_data/SRR11619681_1.fastq --right test_data/SRR11619681_2.fastq
--genome_lib_dir
~/sfa_jaewon/ctat/new5/GRCh37_gencode_v19_CTAT_lib_Mar012021.plug-n-play/ctat_genome_lib_build_dir/
-O peto --cpu 6 --sample_id peto --boosting_method=none
The problem is
[2022-01-11 20:28:44,46] [info] BackgroundConfigAsyncJobExecutionActor
[2aat_mutations.SplitIntervals:NA:1]: executing: /usr/bin/env bash
/PHShome/jst_data/peto/cromwell-executions/ctat_mutations/2a83efc2-5d77-4cde-a0fd-38bb/call-SplitIntervals/execution/script
[2022-01-11 20:28:47,62] [info] BackgroundConfigAsyncJobExecutionActor
[2aat_mutations.StarAlign:NA:1]: job id: 16935
[2022-01-11 20:28:47,63] [info] BackgroundConfigAsyncJobExecutionActor
[2aat_mutations.SplitIntervals:NA:1]: job id: 16952
[2022-01-11 20:28:47,65] [info] BackgroundConfigAsyncJobExecutionActor
[2aat_mutations.SplitIntervals:NA:1]: Status change from - to
WaitingForRetur
[2022-01-11 20:28:47,65] [info] BackgroundConfigAsyncJobExecutionActor
[2aat_mutations.StarAlign:NA:1]: Status change from - to
WaitingForReturnCode
after showing this status, then the pipeline was stuck
I found that from "stdout" in "call-StarAlign/execution"
Jan 11 15:28:44 ..... started STAR run
Jan 11 15:28:44 ..... loading genome
Jan 11 15:32:26 ..... started 1st pass mapping
Jan 11 15:35:28 ..... finished 1st pass mapping
Jan 11 15:35:32 ..... inserting junctions into the genome indices
Jan 11 15:44:03 ..... started mapping
Jan 11 15:47:17 ..... finished mapping
Jan 11 15:47:47 ..... started sorting BAM
Jan 11 15:47:49 ..... finished successfully
I found from my previous result that after Star Alignment, there should be
"call-MarkDuplicates", but currently, there is no "call-MarkDuplicates".
Thank you
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Thank you for your comment |
hi,
you might be overwhelming the system if you're trying to run a lot of
samples locally. For large numbers of samples, you would best use the
cloud such as our Terra workflow:
https://app.terra.bio/#workspaces/ctat-firecloud/ctat-mutations
If you do attempt to run them outside the cloud, be sure to have separate
working directories for each sample. Also, keep a look on your system
resources being used. If your file system gets overwhelmed, or you start
going into swap memory, it'll be trouble.
…On Wed, Jan 12, 2022 at 1:54 PM jaewon-cho ***@***.***> wrote:
Thank you for your comment
But, actually, I have to run more than 10K samples. It is impossible to
kill and rerun everything.
Is there any way not to stick the process? (current job has been stopped
the whole night yesterday)
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The Broad Institute
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|
Thank you for your kind and rapid response. |
I was running the ctat-mutation pipeline with singularity.
I have done the "test" data before
Here is my script:
singularity exec -e -B ${CTAT_GENOME_LIB}:/data/hemberg/jaewon/ctat/new5/GRCh37_gencode_v19_CTAT_lib_Mar012021.plug-n-play/ctat_genome_lib_build_dir/ ~/ctat_mutations.v3.2.0.simg ~/ctat-mutations-CTAT-Mutations-v3.2.0/ctat_mutations --left test_data/SRR11619681_1.fastq --right test_data/SRR11619681_2.fastq --genome_lib_dir ~/sfa_jaewon/ctat/new5/GRCh37_gencode_v19_CTAT_lib_Mar012021.plug-n-play/ctat_genome_lib_build_dir/ -O peto --cpu 6 --sample_id peto --boosting_method=none
The problem is
[2022-01-11 20:28:44,46] [info] BackgroundConfigAsyncJobExecutionActor [2aat_mutations.SplitIntervals:NA:1]: executing: /usr/bin/env bash /PHShome/jst_data/peto/cromwell-executions/ctat_mutations/2a83efc2-5d77-4cde-a0fd-38bb/call-SplitIntervals/execution/script
[2022-01-11 20:28:47,62] [info] BackgroundConfigAsyncJobExecutionActor [2aat_mutations.StarAlign:NA:1]: job id: 16935
[2022-01-11 20:28:47,63] [info] BackgroundConfigAsyncJobExecutionActor [2aat_mutations.SplitIntervals:NA:1]: job id: 16952
[2022-01-11 20:28:47,65] [info] BackgroundConfigAsyncJobExecutionActor [2aat_mutations.SplitIntervals:NA:1]: Status change from - to WaitingForRetur
[2022-01-11 20:28:47,65] [info] BackgroundConfigAsyncJobExecutionActor [2aat_mutations.StarAlign:NA:1]: Status change from - to WaitingForReturnCode
after showing this status, then the pipeline was stuck
I found that from "stdout" in "call-StarAlign/execution"
Jan 11 15:28:44 ..... started STAR run
Jan 11 15:28:44 ..... loading genome
Jan 11 15:32:26 ..... started 1st pass mapping
Jan 11 15:35:28 ..... finished 1st pass mapping
Jan 11 15:35:32 ..... inserting junctions into the genome indices
Jan 11 15:44:03 ..... started mapping
Jan 11 15:47:17 ..... finished mapping
Jan 11 15:47:47 ..... started sorting BAM
Jan 11 15:47:49 ..... finished successfully
I found from my previous result that after Star Alignment, there should be "call-MarkDuplicates", but currently, there is no "call-MarkDuplicates".
Thank you
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