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library(openCyto) library(ggcyto) #> Loading required package: ggplot2 #> Loading required package: flowCore #> Loading required package: ncdfFlow #> Loading required package: BH #> Loading required package: flowWorkspace #> As part of improvements to flowWorkspace, some behavior of #> GatingSet objects has changed. For details, please read the section #> titled "The cytoframe and cytoset classes" in the package vignette: #> #> vignette("flowWorkspace-Introduction", "flowWorkspace") matrix <- matrix(c(17.1702575683594, 3604.7919921875, 4885.357421875, 3255.04663085938, 5461.55712890625, 336.679504394531, 20.1738185882568, 57.9650382995605, 10395.81640625, 3046.4130859375, 15.0239753723145, 36.7612495422363, 3241.30859375, 63.2228126525879, 8053.177734375, 8428.623046875, 29.3448581695557, 3647.14086914062, 30.0510349273682, 4562.94677734375, 7710.4169921875, 50.1847648620605, 8064.9267578125, 35.1330337524414, 6951.19140625, 58.7328872680664, 7004.82666015625, 4567.06640625, 63.8580627441406, 1890.22082519531, 13072.3974609375, 2986.533203125, 8930.966796875, 15.9675989151001, 5982.67529296875, 7193.5205078125, 4095.54418945312, 2986.24951171875, 5685.44580078125, 8338.701171875, 3061.89038085938, 40.2612915039062, 222.174133300781, 3855.7373046875, 52.5284042358398, 2251.46533203125, 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19.3967170715332, 4451.7470703125, 3440.92822265625, 1600.72863769531), ncol = 1 ) colnames(matrix) <- "SSC-H" fr <- flowFrame(exprs = matrix) test_gate_1 <- gate_mindensity2(fr, channel = "SSC-H") ggcyto(fr, aes(x = "SSC-H")) + geom_density() + geom_gate(test_gate_1)
test_gate_2 <- gate_mindensity2(fr, channel = "SSC-H", gate_range = c(0,5000)) ggcyto(fr, aes(x = "SSC-H")) + geom_density() + geom_gate(test_gate_2)
test_gate_3 <- gate_mindensity2(fr, channel = "SSC-H", min = 0, max = 5000) ggcyto(fr, aes(x = "SSC-H")) + geom_density() + geom_gate(test_gate_3)
sessionInfo() #> R version 4.2.2 (2022-10-31) #> Platform: x86_64-apple-darwin17.0 (64-bit) #> Running under: macOS Big Sur ... 10.16 #> #> Matrix products: default #> BLAS: /Library/Frameworks/R.framework/Versions/4.2/Resources/lib/libRblas.0.dylib #> LAPACK: /Library/Frameworks/R.framework/Versions/4.2/Resources/lib/libRlapack.dylib #> #> locale: #> [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 #> #> attached base packages: #> [1] stats graphics grDevices utils datasets methods base #> #> other attached packages: #> [1] ggcyto_1.26.4 flowWorkspace_4.10.1 ncdfFlow_2.44.0 #> [4] BH_1.81.0-1 flowCore_2.10.0 ggplot2_3.4.2 #> [7] openCyto_2.10.1 #> #> loaded via a namespace (and not attached): #> [1] tidyselect_1.2.0 xfun_0.38 lattice_0.21-8 #> [4] colorspace_2.1-0 vctrs_0.6.1 generics_0.1.3 #> [7] htmltools_0.5.5 stats4_4.2.2 yaml_2.3.7 #> [10] utf8_1.2.3 XML_3.99-0.14 RBGL_1.74.0 #> [13] rlang_1.1.0 hexbin_1.28.3 pillar_1.9.0 #> [16] glue_1.6.2 withr_2.5.0 DBI_1.1.3 #> [19] Rgraphviz_2.42.0 BiocGenerics_0.44.0 RColorBrewer_1.1-3 #> [22] plyr_1.8.8 matrixStats_0.63.0 lifecycle_1.0.3 #> [25] zlibbioc_1.44.0 RProtoBufLib_2.10.0 munsell_0.5.0 #> [28] gtable_0.3.3 cytolib_2.10.1 evaluate_0.20 #> [31] labeling_0.4.2 Biobase_2.58.0 knitr_1.42 #> [34] fastmap_1.1.1 flowClust_3.36.1 parallel_4.2.2 #> [37] fansi_1.0.4 Rcpp_1.0.10 scales_1.2.1 #> [40] S4Vectors_0.36.2 graph_1.76.0 farver_2.1.1 #> [43] fs_1.6.1 gridExtra_2.3 digest_0.6.31 #> [46] dplyr_1.1.1 grid_4.2.2 cli_3.6.1 #> [49] tools_4.2.2 magrittr_2.0.3 tibble_3.2.1 #> [52] pkgconfig_2.0.3 data.table_1.14.8 reprex_2.0.2 #> [55] rmarkdown_2.21 rstudioapi_0.14 R6_2.5.1 #> [58] compiler_4.2.2
Created on 2023-04-10 with reprex v2.0.2
The text was updated successfully, but these errors were encountered:
reprex mindensity file (posted on RGLab/openCyto#246)
749af4d
@maximesunnen the outputs look correct to me, we can try to help if you provide additional context or explanation of the expected result in each case.
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Created on 2023-04-10 with reprex v2.0.2
The text was updated successfully, but these errors were encountered: